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KMID : 1137020050160020113
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Journal of Gynecologic Oncology
2005 Volume.16 No. 2 p.113 ~ p.122
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Immunohistochemical study of 14-3-3-sigma and -zeta in ovarian cancer
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Park Ji-Kwon
Shin Jeong-Kyu
Choi Won-Jun
Lee Soon-Ae
Lee Jong-Hak
Paik Won-Young
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Abstract
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OBJECTIVE: To evaluate the expression level, localization and distribution of 14-3-3sigma and zeta proteins in human ovarian epithelial carcinoma cells.
METHODS: Epithelial carcinoma, benign epithelial tumor and normal ovarian tissues were evaluated by immunohistochemistry. For this study, mucinous adenoma (n=10), serous adenoma (n=10), mucinous adenocarcinoma (n=10) and serous adenocarcinoma (n=10) of ovary and normal ovarian tissue (n=10) were analyzed. Goat anti 14-3-3sigma polyclonal antibody and rabbit anti-14-3-3zeta polyclonal antibody were used as primary antibodies.
RESULTS: The 14-3-3sigma protein expression in the glandular epithelium was not statistically different among normal ovary, cystadenoma and cystadenocarcinoma. The 14-3-3zeta protein expression in the glandular epithelium was significantly higher for cystadenocarcinoma than for cystadenoma and normal ovary. The 14-3-3sigma and zeta proteins were immunohistochemically demonstrated in the nuclei and more preferably in the cytoplasm of adenocarcinoma epithelial cells.
CONCLUSION: The expression of 14-3-3zeta protein increased significantly in ovarian adenocarcinoma compared with adenoma and normal ovary. It is suggested that 14-3-3zeta protein expression may play an important role in abnormal growth of ovarian epithelial tumor cell.
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KEYWORD
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14-3-3sigma, 14-3-3zeta, Ovarian epithelial carcinoma, Immunohistochemistry
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